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Alternatively, the APCs may be provided with a means of synthesizing large quantities of the blocking peptides intracellularly. The peptides of the invention may be introduced into APCs directly, e.g., by intravenous injection of a solution containing one or more of the peptides. Because the blocking peptides of the invention are self peptides with the exact carboxy and amino termini tolerized during ontogeny, they are immunologically inert and will not induce an immune response which may complicate treatment using non-self blocking peptides. Once in the APC, a peptide binds to the class II molecules with high affinity, thereby blocking the binding of immunogenic peptides which are responsible for the immune reaction characteristic of the disease condition. It may be useful to employ a “cocktail” of peptides, where complete degeneracy is lacking for individual peptides, i.e., where peptides binds to fewer than all allotypes the cocktail provides overlapping binding specificity. Tissue typing to determine the particular class II alleles expressed by the patient may be unnecessary, as the peptides of the invention are bound by multiple class II isotypes. In the therapeutic methods of the invention, short peptides modelled on the high-affinity immunomodulating self peptides of the invention (which preferably are nonallelically restricted) are introduced into the APCs of a patient. In addition, such therapy could be used to reduce transplant rejection. These three characteristics-(1) self rather than foreign, (2) degeneracy, and (3) high affinity binding-suggest a novel means for therapeutic intervention in disease conditions characterized by autoreactivity, such as Type I diabetes, rheumatoid arthritis, and multiple sclerosis. Furthermore, many if not all of the self-peptides disclosed herein bind to the class II molecules with relatively high affinity. This observation runs counter to the widely-accepted view of MHC class II function, which dictates that each allotype binds a different set of peptides. Several self peptide families have been identified with the unexpected property of degenerate binding: that is, a given self-peptide will bind to a number of HLA-DR allotypes. These peptides were found to be predominantly derived from self proteins rather than foreign proteins. In the work disclosed herein, naturally processed peptides bound to six of the some 70 known human MHC class II DR allotypes (HLA-DR1, HLA-DR2, HLA-DR3, HLA-DR4, HLA-DR7, and HLA-DR8) have been characterized. The amount of each species of peptide which binds class II is determined by its local concentration and its relative binding affinity for the given class II binding groove, with the various allotypes displaying different peptide-binding specificities. Class II MHC molecules bind not only to peptides derived from exogenous (ingested) proteins, but also to those produced by degradation of endogenous (self) proteins. The class II/peptide complex is transported from the endosomes to the cell surface where it becomes accessible to T-cell recognition and subsequent activation of immune responses. In the endosomes, Ii is removed by a process involving proteolysis this exposes the peptide binding cleft, thus allowing peptides present in the endosome to bind to the MHC molecule. Association with Ii is proposed to block the premature acquisition of peptides (by blocking the peptide binding cleft of the MHC heterodimer), promote stable &agr /&bgr interaction, and direct subsequent intracellular trafficking of the complex to endosomal vesicles. Within the ER the class II &agr /&bgr chain complex associates with an lo additional protein termed the invariant chain (Ii). Termini which target them to the endoplasmic reticulum (ER). There were various methods of performing the execution. Constantine the Great, the first Christian emperor, abolished it in the Roman Empire in the early 4th century ce out of veneration for Jesus Christ, the most famous victim of crucifixion. SpaceNext50 Britannica presents SpaceNext50, From the race to the Moon to space stewardship, we explore a wide range of subjects that feed our curiosity about space!Ĭrucifixion, an important method of capital punishment particularly among the Persians, Seleucids, Carthaginians, and Romans from about the 6th century bce to the 4th century ce.Learn about the major environmental problems facing our planet and what can be done about them! Saving Earth Britannica Presents Earth’s To-Do List for the 21st Century.Britannica Beyond We’ve created a new place where questions are at the center of learning.
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The idea here is that this content works as a pastime. Some cities or regions, such as Manhattan and New York City, are presented in 3D, but the traditional Maps view remains applicable to almost all content. I wonder if the connections between Larry Page and East Lansing have something to do with the fact that Ann Arbor still hasn’t been rendered, while East Lansing has been twice.What about drive a car through any city in the world using only google maps? It’s an idea driving simulatorr, created as a plugin for Google Earth – is called 3D driving simulator – and which can be played today in the version below. The scaffolding on the Capitol Dome makes it hard to judge, but the curving corner of the former Knapp’s Department Store (Washington and Washtenaw) is probably worse than the previous render, and the Michigan Hall of Justice on the west end of the state government complex also has issues, to name a few. Curved buildings still seem to be a challenge. The airport lies just outside the new rendering. Airplanes in some cities actually have a tail section that connects to the fuselage, rather than floating in space. Trees are also more realistic, to the extent that you can sometimes tell what kind they are. One thing about the latest renderings is that they actually do stationary cars. Colors seem a bit gaudy (and not just on some of the new apartment buildings, which are, indeed, gaudy). It’s visible from the south, but not the north. There is an interesting phenomenon caused by a moving Pepsi truck on the Shiawassee Street Bridge. It doesn’t cover quite all the area covered by the previous 3D rendering. There are several new buildings and construction projects, as well as the renovation of the Capitol Dome that date it. The imagery is from sometime during the past summer, around the same time as the non-3D was most recently updated (July). Lansing and East Lansing, Michigan have been updated within the last two weeks or less. To see where all the 3D imagery is download our KML file Hong Kong must have been a particularly difficult challenge to photograph, with all its tall buildings and mountains. The Verrazano Narrows Bridge between Staten Island and Brooklyn, New York. One reason for the long processing time is that Google puts extra effort into certain structures such as bridges, as can be seen below. It is hard to put an exact date on most of the 3D imagery, but when we have been able to identify the date the imagery is typically released six months to a year after it was captured. California alone has more 3D than any country in the world other than the US. US states are listed separately as the US has more coverage than the rest of the world. If you are interested in the areas by country, you can download the figures as a. Areas calculated with the help of this site. Google has released a significant number of updates to already existing 3D, but that is harder to track, so we have not attempted to keep a record.Īrea in square kilometres of 3D released by Google. It must be noted also that we are looking at areas not previously covered with 3D or expansions to existing areas. Take the figures as approximate as we found significant discrepancies in our totals suggesting we have some mistakes in our KML map (which we are looking in to). If we got our map and calculations reasonably correct then it seems September was a particularly good month as far as new 3D is concerned. It has been a while since we last looked at the progress of 3D imagery in terms of area covered. [Update: It turns out the September figures are wrong (thank you Frank) due to errors in the KML not being handled well by the Area calculator we used. Make necessary preparations like ratio adjustments or screen lock before you record. It helps you set up your screen before starting. One of the most useful features Camtasia has is the mini toolbar that pops out in record mode. You can capture your whole screen or just a part of it. This is useful for business and personal projects. Recording your screen is the main purpose of CS, so it’s super easy to do. That puts a limit on your creativity as you’d have to find ways to not make your videos look like a default. You can only customise certain effects up to some degree. One thing is that they’re all fundamental. The effects work well, and their implementations are also smooth. Make them dance or splash into a title with the behavior tool. You can also customise the texts in your clip. These involve a lot of colours and shadowing that give your content a custom atmosphere. If you want extra enhancement for your video, there are some visual effects you can play with. It has all the popular transitions, including the blur and pixelate. With this tool, you can decide how each of your scenes comes into view. There are also many transitional effects to choose from. Each effect comes with customisable layouts like colors and density. Add clarifying texts or subtitles to your video with the annotations tool. Make your clips spin or fade into view with the opacity and tilt animations. The ones it does have are still good and will fulfil most of your small projects’ needs. Since Camtasia caters to new and intermediate users, you won’t find a large selection of special effects. Videos exported from the free trial version will contain the Camtasia Studio watermark. This gives you a chance to fully test the software and see if it’s right for you. During this trial, you’re able to access all the same features as you would in the full version. Is Camtasia Studio free?Ĭamtasia Studio is not a free program, but it does have a free trial. You will want to learn all the technical terms first. One slight setback to learning by yourself is that the buttons don’t have clear instructions on them. So, if you’re not keen on watching tutorials, it wouldn’t take long to figure everything out. Other programs, such as OBS Studio or Filmora Video Editor, can do the same thing, but not as easily as CS. It’s an inherent mechanic that lets you combine multiple clips into one video. So, you can try all the game-changing features such as the overlapping media. The good thing about Camtasia is that the free version contains every function of the original one. Keep in mind that if you’re using the free trial, there will be a watermark on your content until you upgrade. Use CS for anything from YouTube videos and tutorials to gaming sessions. Creative projects made simpleĬamtasia records your entire screen, so the range of content you can create is extensive. There’s a voice-over function that lets you narrate your process. You can also enhance your videos with special effects or annotations with the easy to use tools. Use this app to record activities on your screen for demonstration or promotional purposes. Camtasia Studio by TechSmith is a video and screen recorder for Windows that captures your screen or webcam. The Good Stuff: Whatever you do, work heartily, as for the Lord and not for men, knowing that from the Lord you will receive the inheritance as your reward. Read “My happiness is the most important thing about my marriage” and seven other lies that destroy marriage. Perhaps a lack of hyperfocus on our own happiness could also contribute to compassion for our partner long after the veil’s lost in the attic.īut if we see a fulfilling marriage as an uphill battle from the start, something precious and hardwon, a commitment upheld by two families-there’s something sacred to glean. Especially when the modern standard of worthwhile marriage seems to fall along the lines of “Am I happy?” We venture into marriage stocked with happy love hormones … that scientifically last a maximum of 18 months. What I learn from arranged marriages (not that I’m endorsing arranged marriages, Lifetime, child brides, or anything else you might dislike)? It’s some version of plan to work-and with community-for your happy marriage.Īnd perhaps, have expectations compatible with planet Earth. an eight-part reality series that would propel desi arranged marriages onto a. On the show, singles entrust matrimony to experts, who use compatibility and coaching for a new generation of matchmaking.Īrranged marriages seem to view marriage as a family-supported event, rather than the mere responsibility of two individuals. Indian Matchmaking at Emmys is big recognition - of the show, yes. 2020 Maturity Rating: TV-MA 1 Season TV Shows Based on Books. The docu-series follows otherwise modern American singles who share the tradition of arranged marriage. Joseph Cilona, weighing in on Lifetime TV show, Married at First Sight.** The second season of FYI’s Arranged TV series premieres Tuesday, at 9:00pm ET/PT. This often means that commitment to working through issues is often put before personal needs and feelings,” explains psychologist Dr. “What makes arranged marriages work can be summed up in one word: Commitment. And arranged-marriage partners are nearly twice as compassionate to each other 10 years in.* Yet there’s actually no statistical difference in happiness between arranged and love-/choice-based marriages. In an era where people are more bent towards looking for their own partners instead of opting for the traditional arranged marriage route, there are stories that show us how the concept of arranged marriage, with a lot of evolvement, can be a happy space too. Letting my family pick my husband? Singleness would look better and better. You, Too, Can Learn From Arranged MarriagesĪs a woman-with a decent degree of independence from my parents-the idea of an arranged marriage punctures me with fear. I fiddled with - recast - for a couple of minutes, but then, well, I failed. I do believe we should not use SEs where SDs or CIs are better applicable, for that would be taken as "photoshopping" the results, possibly out of vested interests, who knows.Īlso amazing enough, when preparing my demonstration, I kept for quite a while wondering whether I could successfully - recast - the graphic as a bar graph, like the one Nick shared above. The reason for this, also guessed correctly by Nick, is precisely my despise of it when applying for situations where the SD should be selected instead (well underlined in a) and b) in #6. No wonder, he guessed my thoughts right, even when remarking about the - se - I should have demonstrated as well, for I preferred to choose the SD in the example I shared.Īctually, at first, my example presented both options, but I decided to avoid showing the SE for what I fear to be a somewhat misleading presentation in this particular case. Nick, as always, provided an insightful reply concerning the matter. I was curious about whether Marcos' example could be adapted to produce the execrable detonator or dynamite plots for consenting adults, and it can. Straight estimate +/- se or estimate +/- 2 * se bars seem to be used for one of more of the following reasons (a) tribal habit in some scientific fields (b) they look better in understating uncertainty (especially +/- se) (c) they are approximations to more precisely calculated confidence intervals. I suspect that the more statistically minded you are, the more you want confidence intervals. There is a larger and different issue that, on the whole, the users are ahead of the developers in programming what they want under this heading. It seems a bit untidy that you need collapse to produce sd (or semean, as Marcos could have shown) and ci to produce confidence intervals at specified confidence levels such as 95%, but dotplot, bar should be mentioned too. I just care here about how easy Stata is to use and the original post I think is guilty of exaggeration on this point. I (we, presumably) don't mind other programs being easy to use when they are. I think Bruce's example shows that SPSS is a bit (no, a lot) simpler here. The first is from the GRAPH command, the second from GGRAPH (i.e., the Chart Builder). Here are the plots showing means with 95% CIs. GUIDE: text.footnote(label("Error Bars: +/- 1 SD"))ĮND GPL.Note that all of these commands use the raw data. GRAPHDATASET NAME="graphdataset" VARIABLES=Foreign MEANSD(mpg, 1)[name="MEAN_mpg" * Plot mean & bars extending 1*SD below and ablve. GUIDE: text.footnote(label("Error Bars: +/- 1 SE")) GRAPHDATASET NAME="graphdataset" VARIABLES=Foreign MEANSE(mpg, 1)[name="MEAN_mpg" * Plot mean & bars extending 1*SE below and ablve. GUIDE: text.footnote(label("Error Bars: +/- 2 SE")) GRAPHDATASET NAME="graphdataset" VARIABLES=Foreign MEANSE(mpg, 2)[name="MEAN_mpg" * Plot mean & bars extending 2*SE below and ablve. GUIDE: text.footnote(label("Error Bars: 95% CI"))ĮLEMENT: interval(position(Foreign*MEAN_mpg), shape.interior(shape.square))ĮLEMENT: interval(position((Foreign*(LOW+HIGH))), shape.interior(shape.ibeam)) GUIDE: text.title(label("Simple Bar Mean of mpg by Foreign")) LOW="MEAN_mpg_LOW" HIGH="MEAN_mpg_HIGH"] MISSING=LISTWISE REPORTMISSING=NOĭATA: Foreign=col(source(s), name("Foreign"), unit.category())ĭATA: MEAN_mpg=col(source(s), name("MEAN_mpg"))ĭATA: LOW=col(source(s), name("MEAN_mpg_LOW"))ĭATA: HIGH=col(source(s), name("MEAN_mpg_HIGH")) GRAPHDATASET NAME="graphdataset" VARIABLES=Foreign MEANCI(mpg, 95)[name="MEAN_mpg" * Watch for the following functions: MEANCI(), MEANSE(), MEANSD(). * The new-fangled "Chart Builder" allows greater flexibility (at the cost of more code). GRAPH /ERRORBAR(STDDEV 1)=mpg BY Foreign. GRAPH /ERRORBAR(STERROR 1)=mpg BY Foreign. GRAPH /ERRORBAR(STERROR 2)=mpg BY Foreign. Code: * The "legacy" GRAPH command in SPSS. *Not recognized as an essential nutrient by the AAFCO Dog Food Nutrient Profiles. 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